ABSTRACT
Objective: To examine the stem cell-like properties of dormant cells in ovarian epithelial carcinoma transplantation tumors. Methods: The PKH26-labeled human ovarian cancer SKOV3 cells were inoculated subcutaneously in nude mice. According to the fluorescence intensity of PKH2, the tumor cells were divided into 3 groups using FCM method: PKH26 cells with high fluorescence retention (PKH26hi cells), PKH26 cells with low fluorescence retention (PKH26low cells), and PKH26 cells with fluorescence quenched completely (PKH26neg cells). The cell cycle of PKH26hi, PKH26low and PKH26neg SKOV3 cells was detected by FCM method. The mRNAs and proteins of stem cell markers octamer-binding protein 3/4 (OCT3/4), CD1 33, CD1 77 and Nanog were examined by real-time fluorescence-based quantitative PCR and Western blotting. The colony forming ability of PKH26hi, PKH26low and PKH26neg SKOV3 cells in vitro was observed by plate colony formation assay, and the tumorigenic ability in vivo was observed in nude mice. Results: The percentage of G0/G1 cells in PKH26hi SKOV3 cells was higher than those in PKH26low and PKH26neg cells (both P < 0.05). The mRNA and protein expression levels of stem cell markers OCT3/4, CD1 33, CD1 1 7 and Nanog in PKH26hi were higher than those in PKH26low and PKH26neg SKOV3 cells (all P < 0.05). The abilities of in vitro colony formation of PKH26hi SKOV3 cells and their tumorigenesis in nude mice were significantly higher than those of PKH26low cells and PKH26neg SKOV3 cells (both P < 0.05). Conclusion: PKH26hi cells in ovarian cancer SKOV3 cell xenografts in nude mice have stem cell-like properties.
ABSTRACT
Prior to the formation of new metastases,a large number of cancer cells released from the primary tumor enter the dormancy period.Once the dorruancy is broken,tumor cells will recover the capability of proliferation.In recent years,various hypotheses and mechanisms of tumor metastasis have been studied.Primary tumor resection is considered to be an important factor to break tumor dormancy state.It is recognized as an important reason to promote tumor metastasis.The improvement of surgical technique and further study on the mechanism of dormancy may provide new ideas for the treatment of metastatic tumor.
ABSTRACT
Tumor dormancy is a phase of occurrence and progress of cancer.In recent years,with the further study of circulating tumor cells,disseminated tumor cells and cancer stem cells,it is found that they are closely related to the tumor dormancy.Moreover,tumor microenvironment is another important factor in tumor dormancy.
ABSTRACT
Tumor dormancy is a state that tumor cells long-term exist in human body,without obvious growth. Dormant cell is one of the origins of tumor recurrence or metastasis. The exact mechanism of tumor dor-mancy is still unclear. Combined with cellular and molecular biological techniques,tumor cell transplantation and culture in vitro can establish experimental tumor dormancy-recurrence models. These in vivo and in vitro models can be used to investigate mechanisms of tumor dormancy and to explore novel treatments.